Biliatresone regulates RhoU/Wrch1, Hey2 and Sox17 expression in cholangiocytes. Primary mouse neonatal extrahepatic cholangiocyte spheroids were treated with biliatresone (2 ug/ml), BSO (100 µM), or DMSO (same volume as biliatresone) for 24 h. Representative images of spheroids immunostained for F-actin (red), and RhoU/Wrch1 or Hey2 (magenta). Nuclei were stained with DAPI (blue). Images are representative of n=4 independent experiments. Scale bar = 10 µm.