Experimental Hematology

המעבדה להמטולוגיה ניסויית
Head of the lab:
Prof. Pia Raanani, M.D
Phone: 03-9377905, 03-9377906
Lab Manager:
Dr. Galit Granot, PhD
Phone: 03-9376795, 052-8368829
Research team:
    ד"ר איילה גובר-פרואקטור    
    ד"ר אושרת רז    
    ד"ר סער שפירא    
    ד"ר אלונה טלרמן    
    ד"ר מיכאל ענבר    
Students:
    עלא אלדין סמארה (תואר שלישי)    
Research Areas:

Our Lab is for Translational Research in Hematology. Its main strength is the tight collaboration and involvement of physicians from the Institute of Hematology at the Davidoff Cancer Center, Rabin Medical Center. Our primary field of interest is finding new or better therapies for the treatment of incurable hematological malignancies. Our projects focus on understanding the effect of new agents on different leukemia and lymphoma cell lines and patient samples and exploring the deleterious side effects caused by customary agents used today. In addition, we are studying the molecular pathways involved in the initiation and maintenance of hematological tumorigenesis and are trying to understand the effect of the different agents on these molecular pathways.

We apply cutting-edge technologies including molecular, protein and cellular biology, confocal microscopy, live cell imaging, NGS analyses, cell line and murine models etc.

Research Topics

Pathogenesis of tyrosine kinase inhibitor (TKI) associated vascular disease in chronic myelocytic leukemia (CML).

Ponatinib inhibits HUVEC tube formation. HUVECs plated on matrigel-coated plates in the presence of different TKIs. Tube formation was evaluated by light microscopy. Pictured are Representative fields (×4 mag.) of phase-contrast and fluorescent calcein-stainings.
Gover-Proaktor A et al Ponatinib reduces viability, migration, and functionality of human endothelial cells. Leuk Lymphoma. 2017;58(6):1455-1467.

Increased extrusion of DNA strands and H3cit expression in CML neutrophils. Immunofluorescent images of CML patient vs. healthy, matched control neutrophils. DNA strands were stained with DAPI and histone H3cit was stained with H3cit specific antibody. Cells were observed by Apotome microscope (X40).
 

NETosis in CML and the effect of tyrosine kinase inhibitors (TKIs) on NETosis.

CLL cells exhibit a different circRNA profile compared to normal B cells. Supervised hierarchical clustering displaying 87 differentially expressed genes (36 downregulated and 51 upregulated) in CLL cells compared to normal B cells using a fold-change difference cutoff of 3-fold and FDR 1x10-5

The role of circular RNA (circRNA) in chronic lymphocytic leukemia (CLL).

 Engineering of exosomes to target specific cancer cells.

  • Mechanism of the anti-tumoral activity of deferasirox on mantle cell lymphoma.

 

Publications:
Contact details
Granot Galia Mobile: 052-8368829
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